Salt-Dependent Aggregation and Assembly of E coli-Expressed Ferritin

نویسندگان

  • Wei Sun
  • Chengfeng Jiao
  • Yue Xiao
  • Luowei Wang
  • Cheng Yu
  • Jialin Liu
  • Yongli Yu
  • Liying Wang
چکیده

Ferritin, with the primary function of iron storage, is a nearly ubiquitous protein found in most living organisms. Our recent investigations suggest that ferritin can assemble nanoparticles. So we use ferritin as a novel type of delivery vehicle for recombinant epitope vaccines. And, we found that ferritin form nonnative aggregates depended sensitively on NaCl concentrations. Here, we report that ferritin is an ion-sensitive protein and has the attribute of salt-dependent aggregation. Our results indicate that recombinant ferritin can be released as a soluble form from Escherichia coli at low NaCl concentrations (≤50 mmol/L). Moreover, this result affords us to confirm a proper self-assembling solution for soluble ferritin or other ferritin-based fusion proteins to assemble nanoparticles.

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عنوان ژورنال:

دوره 14  شماره 

صفحات  -

تاریخ انتشار 2016